Microwave synthesis of blue emissive carbon dots from 5-sulpho anthranilic acid and 1,5-diphenyl carbazide for sensing of levocetirizine and niflumic acid.

In this work, water soluble carbon dots (CDs) were synthesized by using 5- sulpho anthranilic acid (SAA) and 1,5-diphenylycarbazide (DPC) as precursors via microwave-assisted method and named as "SD-CDs". We studied the effect of SAA and DPC molar ratio (1:3, 2:2 and 3:1) for the preparation of blue fluorescent CDs, showing the best emission properties at molar 3:1 ratio of SAA and DPC. The as-prepared SD-CDs emit bright blue color under UV light at 365 nm, and exhibit emission peak at 392 nm when excited at 319 nm. The as-synthesized SD-CDs act as a fluorescent sensor for detection of levocetirizine and niflumic acid through the fluorescence "turn-on-off" mechanism. The developed probe exhibited good linearity in the concentrations (levocetirizine - 1.0-100 µM and niflumic acid - 0.5-100 µM) with detection limits of 3.92 nM and 0.19 µM for levocetirizine and niflumic acid, respectively. Importantly, the developed analytical method was successfully used for the detection of levocetirizine in tablets and niflumic acid in biofluids of human (serum, plasma and urine), showing good recoveries from 97 to 99 %. Thus, this SD-CDs-based fluorescence method has the potential for levocetirizine and niflumic acid assays in biological and pharmaceutical samples.