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CdSe/ZnS core shell quantum dot-based FRET binary oligonucleotide probes for detection of nucleic acids.

PubMed

Authors: Peng Y, Qiu C, Jockusch S, Scott AM, Li Z, Turro NJ, Ju J

Year

2012

Paper ID

12218

Status

Peer-reviewed

Abstract Read

~2 min

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Abstract

We report the design, synthesis, and characterization of a binary oligonucleotideprobe for selective DNA or RNA detection. The probe is based on fluorescence resonance energy transfer (FRET) from quantum dot (CdSe/ZnS core shell) DNA conjugates to organic dye (cyanine-5) DNA conjugates. Selective hybridization of the donor/acceptor DNA conjugates to target DNA enhances FRET and a change in fluorescence signature was observed.

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We report the design, synthesis, and characterization of a binary oligonucleotideprobe for selective DNA or RNA detection.

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References & Citation Signals

[1] DOI https://doi.org/10.1039/c1pp05132f [2] Publisher https://pubmed.ncbi.nlm.nih.gov/21874193/

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