Luminous Insights: Harnessing Carbon Nanodots from Black Seed Powder via Pyrolysis for Bioimaging and Antifungal Investigations.

This study delves into the fabrication of carbon nanodots (CNDs) through a bottom-up approach, utilizing black seed powder as the precursor material and employing the pyrolysis method. CNDs were synthesized across four distinct temperature settings. The investigation encompasses an extensive characterization of the CNDs, including optical and structural attributes. UV-visible and fluorescence spectroscopy were utilized to assess their optical properties, while FT-IR and XRD analyses confirmed their structural integrity. To elucidate size, shape, and nature, HR-TEM imaging was employed. Furthermore, the functional applications of the synthesized CNDs were explored. The material's antifungal potential was evaluated, and its viability for bioimaging was demonstrated by successfully labeling yeast cells with CNDs. This study underscores the multifaceted nature of CNDs, serving as a bridge between synthesis, comprehensive characterization, and practical applications. In summary, the investigation provides insights into the versatile applications of CNDs derived from black seed powder through pyrolysis. The study contributes to the understanding of their fundamental properties and establishes their potential for both antifungal treatments and cellular bioimaging.